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The Western Blot test is used for many different human and veterinary medicine tests. It is used to identify antibodies that determine the presence of such conditions as HIV, Hepatitis B, Lyme Disease, Avian Influenza or bird flu and others. FIV, or feline "AIDS", can also be detected using this type of testing.
This type of test, sometimes called Western Blot Protocol, produces a result on film that shows a series of bands. Performing an exacting amount of steps in the exact order produces this result. If the test result shows nothing, the scientist has not only wasted time but has frustration because there are many things that could have gone wrong to produce this result.
While the [url=http://www.vivid-host.com/barbour.htm]barbour uk[/url] scientist can be tempted to run the test again, this is usually the wrong choice because it is likely to give the exact same result. Instead of doing this, the scientist should attempt to rerun the test in stages. Each step can reveal what exactly happened to cause the blank film. By allocating time that is appropriate for each step and carrying it out, it is possible to find out what went wrong. Western blot protocols are very specific on how they are to be accomplished. One misstep in the process can prevent the results from being accurate.
The scientist should first check for problems between [url=http://www.gotprintsigns.com/uggpascher/]bottes ugg pas cher[/url] the primary and secondary antibodies and the blocking agent. A cross reaction here is usually the culprit. Following the steps to do this will result in one, two or completely missing dots on the end result. Getting a positive control dot on the [url=http://www.orlando-apts.com/nfljerseys/]nfl jerseys[/url] film means [url=http://www.1855sacramento.com/peuterey.php]peuterey outlet[/url] its time to replace the antibodies as they have spoiled. Two dots on the film mean this cause can be eliminated. A no dots result points to the ECL solution as the problem.
If this step fails to provide answers, [url=http://www.1855sacramento.com/peuterey.php]giubbotti peuterey[/url] the antibodies have to be tested for compatibility. The results for this type of test can point to one antibody [url=http://www.gotprintsigns.com/hollisterpascher/]hollister france[/url] or [url=http://www.gotprintsigns.com/monclerpascher/]moncler pas cher[/url] the buffer being the problem. Steps for Western Blot solutions continue through a series of steps after this if [url=http://www.lcdmo.com/hollister.php]hollister co france[/url] this has not [url=http://www.achbanker.com/home.php]hollister[/url] been shown to be the cause of blank film. A bad primary antibody, [url=http://www.seattlesoycandles.com]hollister[/url] bad film, bad membranes, buffers or blocking buffer can all be causes. The only way to find out is to eliminate each possibility one at a time.
This type of testing is used for many reasons. However, the Western Blot test [url=http://www.rtnagel.com/airjordan.php]nike air jordan pas cher[/url] is useful only when performed in the right order and the results are correct.
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